Floral Induction of Vegetative Plants Supplied a Purified Fraction of Deoxyribonucleic Acid from Stems of Flowering Plants
- 1 December 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 60 (6), 885-891
- https://doi.org/10.1104/pp.60.6.885
Abstract
It was found that floral induced stems of flowering tobacco (Nicotiana tabacum cv. Wis. 38) plants contain large amounts of rapidly renaturing DNA, whereas non-induced stems of vegetative plants contain only small amounts. The striking qualitative difference in DNA between stems of flowering and vegetative plants mimics the over-all quantitative difference in DNA content (on a fresh weight basis). Therefore, the extra DNA in stems of flowering plants seems, at least in part, to represent preferential synthesis of rapidly renaturing DNA. Rapidly renatured (flowering plants) was purified (cesium chloride gradients) from heated-cooled DNA solution and under non-inductive conditions was tested for floral activity. When rapidly renatured DNA in buffer solution is supplied to axillary vegetative buds of vegetative plants and then the axillary buds are defoliated every 4th day for 12 days, the treated buds change into flower buds. Control axillary buds supplied buffer solution alone remain vegetative. In stem segments from flowering plants, the concept, discussed in previous reports, that IAA may modify in vitro bud expression by directly affecting DNA synthesis was reviewed. On the basis of this report, the concept is elaborated by proposing that IAA may act partially in bud expression by directly suppressing synthesis of rapidly renaturing DNA.This publication has 13 references indexed in Scilit:
- Floral Activity in Solutions of Deoxyribonucleic Acid Extracted from Tobacco StemsPlant Physiology, 1976
- Rapid Initiation of Thymidine Incorporation into Deoxyribonucleic Acid in Vegetative Tobacco Stem Segments Treated with Indole-3-acetic AcidPlant Physiology, 1975
- Flower Formation in Excised Tobacco Stem SegmentsPlant Physiology, 1973
- Purification and Further Properties of Single‐Strand‐Specific Nuclease from Aspergillus oryzaeEuropean Journal of Biochemistry, 1973
- Estimation of the double-helical content in various single-stranded nucleic acids by treatment with a single strand-specific nucleaseBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972
- Inhibition of chloroplast DNA synthesis by cycloheximideJournal of Molecular Biology, 1971
- Flower Formation in Excised Tobacco Stem Segments; II. Reversible Removal of IAA Inhibition by RNA Base AnaloguesPlant Physiology, 1969
- Flower Formation in Excised Tobacco Stem Segments; I. Methodology and Effects of Plant HormonesPlant Physiology, 1969
- The strands of DNA from lambda and related bacteriophages: Isolation and characterizationJournal of Molecular Biology, 1968
- THE BUOYANT BEHAVIOR OF VIRAL AND BACTERIAL DNA IN ALKALINE CsClProceedings of the National Academy of Sciences, 1963