LR white resin and improved on-grid immunogold detection of vicilin, a pea seed storage protein

Abstract

LR White resin combined with post-embedding immunogold labelling was used to localize the storage protein vicilin within developing pea seed cotyledons by electron microscopy. Fine structural preservation is comparable to that obtained with Spurr's resin, and antibody labelling is improved. More gold binds to protein bodies, to rough endoplasmic reticulum and to Golgi vesicles, and in addition, vicilin was detected within Golgi cisternae, a site not previously observed.