Optimal Leukocyte Removal from Refrigerated Blood with the IBM 2991 Blood Cell Processor

Abstract
One hundred forty-six units of 2-5 day old refrigerated blood were washed with the IBM Blood Cell Processor. The [human] ABO and Rh types of the units varied according to available inventory. This study was designed to select the best protocol from the 42 possible combinations utilizing the machine''s variable red blood cell override (RCO). A 1-l wash program was used. The 2 parameters selected for study were the per cent red blood cell recovery and the per cent white blood cell removal. The protocol of choice uses a RCO of 4 in the 1st and 2nd steps of the program. This protocol yields a washed unit with a mean red blood cell recovery of 82% and a mean white cell removal of 93%. The IBM Blood Cell Processor can be used to wash 2-5 day old refrigerated blood for the preparation of leukocyte-poor blood that would meet the Standards of the AABB [American Association of Blood Banks].