Stimulation of Guanosine 3′,5′-Monophosphate Accumulation in Anterior Pituitary Glandsin Vivoby Synthetic Luteinizing Hormone-Releasing Hormone
- 1 February 1980
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 106 (2), 626-630
- https://doi.org/10.1210/endo-106-2-626
Abstract
The in vivo effect of an iv injection of synthetic LHRH on cAMP and cGMP concentrations in the anterior pituitary gland of pentobarbital (31.5 mg/kg BW)-injected proestrous rats and ovariectomized estradiol (10 μg)-primed rats was studied. At a specific time after an injection of LHRH or saline (as the control), the animals were sacrificed by microwave irradiation to the head. LHRH (100 ng/100 g BW) injection in the proestrous rat caused a significant increase in the cGMP level from 10-20 min associated with an increase in the serum LH level, whereas it exerted no effect on cAMP. No changes were observed in saline-injected animals. In the ovariectomized estradiol- primed rat, LHRH (50 ng/100 g BW) injection performed under ether anesthesia also induced a significant elevation of cGMP level, after 5-10 min, in parallel with an elevation of serum LH level. Saline injection caused no change in cGMP. cAMP concentrations were increased 5-10 min after either LHRH or saline injection. These results suggest the involvement of a guanylate cyclase-cGMP mechanism in the mediation of LHRH action in the pituitary. (Endocrinology106: 626, 1980)Keywords
This publication has 3 references indexed in Scilit:
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