Immunoassay of Parathyroid Hormone by Quantitative Complement Fixation12

Abstract

An immunochemical assay method for bovine parathyroid hormone utilizing quantitative complement fixation is described in detail. Compared with biological assay methods generally and, specifically, the Munson method (serum calcium level in acutely parathyroidectomized rats), the new immunoassay method is more precise (λ =0.11), it is simpler to perform, and it can measure lower concentrations of hormone—as little as 24 rang (84 mU) /ml. Immunoassays and bio-assays of parathyroid hormone preparations of widely varying potency were in good agreement. Following storage at – 20 C in dilute neutral solution (Endocrinology74: 244, 1964)