New monoclonal antibodies reacting with bile ducts: Further insights into the pathogenesis of bile ductular proliferation in biliary diseases
- 1 October 1991
- journal article
- Published by Wiley in The Journal of Pathology
- Vol. 165 (2), 153-161
- https://doi.org/10.1002/path.1711650211
Abstract
We have produced a range of monoclonal antibodies which stain human intrahepatic bile ducts of different sizes. Amongst 26 monoclonal antibodies produced, five clones reacted specifically with bile ducts of different sizes, of which three have been maintained in culture and their viability following freezing and thawing confirmed. Staining patterns varied between normal adult liver tissue, normal fetal liver tissue and a variety of hepatobiliary diseases. The antibodies provide further evidence of the immunological heterogeneity of the human intrahepatic biliary tree and support the hypothesis that proliferating bile ductules are derived from periseptal hepatocytes. The preparation of the antiboides, their staining reactions in normal adult, normal fetal and a variety of liver diseases are described.Keywords
This publication has 22 references indexed in Scilit:
- The autoepitope of the 74-kD mitochondrial autoantigen of primary biliary cirrhosis corresponds to the functional site of dihydrolipoamide acetyltransferase.The Journal of Experimental Medicine, 1988
- Blood group antigens in the intrahepatic biliary treeJournal of Hepatology, 1988
- Primary biliary cirrhosis and mitochondrial autoantigens—insights from molecular biologyHepatology, 1988
- Differential expression of keratin 19 in normal human epithelial tissues revealed by monospecific monoclonal antibodiesJournal of Molecular Histology, 1986
- Intrahepatic bile ducts under the lensJournal of Hepatology, 1985
- Patterns of Expression and Organization of Cytokeratin Intermediate FilamentsAnnals of the New York Academy of Sciences, 1985
- The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cellsCell, 1982
- Derivation of specific antibody‐producing tissue culture and tumor lines by cell fusionEuropean Journal of Immunology, 1976
- Continuous cultures of fused cells secreting antibody of predefined specificityNature, 1975
- Selection of Hybrids from Matings of Fibroblasts in vitro and Their Presumed RecombinantsScience, 1964