Syringe method for stepwise chemical synthesis of oligonucleotides
Open Access
- 25 May 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (10), 3249-3259
- https://doi.org/10.1093/nar/10.10.3249
Abstract
A simple procedure is described for synthesis of oligonucleotides by phosphite chemistry. Chains can be constructed rapidly with minimal equipment (a syringe and reagent bottles). The method is illustrated by synthesis of d-TGCAGGTT. Pertinent supporting data on the effect of variations in the detritylation, condensation, oxidation, capping and cleavage steps in the synthetic approach and in isolation procedures are also presented.Keywords
This publication has 7 references indexed in Scilit:
- Automated Synthesis of Gene FragmentsScience, 1981
- Rapid synthesis of oligodeoxyribonucleotides VI. Effident, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester routeNucleic Acids Research, 1981
- Development in the phosphite-triester method of synthesis of oligonucleotides.1980
- [57] Sequencing end-labeled DNA with base-specific chemical cleavagesMethods in Enzymology, 1980
- Phosphorylation of tRNA by T4polynudeotide kinaseNucleic Acids Research, 1977
- Synthesis of thymidine oligonucleotides by phosphite triester intermediatesJournal of the American Chemical Society, 1976
- Solid support synthesis of ollgothymidylates using phosphorochloridates and 1-alkylimidazolesNucleic Acids Research, 1975