MONOIODOINSULIN SPECIFICALLY SUBSTITUTED IN TYR A14 OR TYR A19

Abstract

Porcine monoiodoinsulin was prepared using ion exchange chromatography. The isolated monoiodoinsulin showed on polyacrylamide gel electrophoresis 2 bands with different intensities related to the initial method of iodination. Each of the 2 bands were isolated from the gel, and determination of the I distribution among the tyrosyl groups showed that 1 band contained monoiodoinsulin substituted in Tyr A19 contaminated with monoiodoinsulin substituted in the B-chain. The other band contained essentially A14 monoiodoinsulin. Polyacrylamide gel electrophoresis is a convenient method to prepare homogeneous A14 monoindoinsulin with biological activity indistinguishable from that of native insulin.