SEPARATION OF THE VALYL-LEUCYL- AND VALYL-GLUTAMYL-POLYPEPTIDE CHAINS OF HORSE GLOBIN BY FRACTIONAL PRECIPITATION AND COLUMN CHROMATOGRAPHY
- 1 March 1959
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry and Physiology
- Vol. 37 (3), 405-416
- https://doi.org/10.1139/o59-042
Abstract
Horse globin has been fractionated into two components by stepwise addition of acid acetone to an acidified globin solution and by gradient elution with urea from a carboxylic-type ion exchange resin at low pH. The components obtained by both methods are similar to those prepared earlier by electrophoresis. They have similar molecular weights (about 16,000) and appear to be present in nearly equal amounts. One has the N-terminal sequence valyl-leucyl- and the other valyl-glutamyl-. They differ electrokinetically and in other properties.Keywords
This publication has 11 references indexed in Scilit:
- The N-Terminal Sequence of the β Chains1 of Normal Adult Human HemoglobinJournal of the American Chemical Society, 1958
- ON DISSOCIATION AND RECOMBINATION OF HUMAN ADULT HEMOGLOBINS A, S, AND CProceedings of the National Academy of Sciences, 1958
- On the fractionation of thymus histoneArchives of Biochemistry and Biophysics, 1956
- APPLICATION OF THE ARCHIBALD ULTRACENTRIFUGAL PROCEDURE TO LYSOZYME AND APURINIC ACID: EVALUATION USING A MECHANICAL INTEGRATORCanadian Journal of Chemistry, 1956
- A Paper Chromatographic Method for the Quantitative Estimation of Amino-AcidsNature, 1954
- Reversible association processes of globular proteins. I. InsulinArchives of Biochemistry and Biophysics, 1952
- Ultraviolet Absorption Spectra of Proteins and Amino AcidsAdvances in protein chemistry, 1952
- The separation of N-2:4-dinitrophenyl amino-acids on paper chromatogramsBiochemical Journal, 1951
- Light Scattering in Protein SolutionsAdvances in protein chemistry, 1951
- Some Peptides from InsulinNature, 1948