EFFECT OF LIPID PEROXIDATION ON PHOSPHOLIPASE A2 ACTIVITY OF RAT LIVER MITOCHONDRIA

Abstract
Effect of lipid peroxidation on phospholipase A2activity vas examined in rat liver mitochondria. Content of lipid peroxides in rat liver mitochondria was markedly increased in the presence of ascorbic acid (0.1 mM) and ferrous ion (40 μM), as compared to the control. Phospholipase A2 activity in mitochondria vsas activated by approximately 60% after lipid peroxidation. Mg2+-activated ATPase activity in mitochondria was markedly stimulated by about 200% following treatment with ascorbic acid and ferrous ion. There was no difference in calcium content of mitochondria before and after lipid peroxidation.

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