TAM selection of Drosophila somatic cell hybrids
- 1 January 1979
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 5 (1), 29-37
- https://doi.org/10.1007/bf01538784
Abstract
The selection of MDR3, an adenine-salvage-deficient variant of the Kc line, is described. It is resistant to methylpurine and to diaminopurine and is TAM (thymidine, adenine, methotrexate) sensitive. Two wild-type (TAM-resistant) cell lines, Schneider's line 3 (S3) and Dübendorfer's line 1 (D1), due to their different nutritional requirements, are unable to proliferate in medium ZH1% used for line MDR3. This allowed the selection of hybrids between MDR3 and either D1 or S3 in TAM cloning medium after treatment with polyethyleneglycol. Hybrids were identified by the isoenzyme pattern of NADP-dependent isocitrate dehydrogenase.This publication has 12 references indexed in Scilit:
- Cell fusion between temperature-sensitive mutants of aDrosophila melanogaster cell lineSomatic Cell and Molecular Genetics, 1978
- Isozyme and allozyme patterns in embryonic Drosophila cell culture linesBiochemical Genetics, 1977
- Purine and pyrimidine salvage in a clonal Drosophila cell lineJournal of Insect Physiology, 1977
- GENE EXPRESSION IN SOMATIC CELL HYBRIDSAnnual Review of Genetics, 1974
- Purine metabolism pathways in Drosophila cells growth “in vitro” : Phosphoribosyl transferase activitiesBiochimie, 1974
- Enzyme electrophoresis on cellulose acetate gel: Zymogram patterns in man-mouse and man-Chinese hamster somatic cell hybridsArchives of Biochemistry and Biophysics, 1971
- A New Reduced Human-Mouse Somatic Cell Hybrid Containing the Human Gene for Adenine PhosphoribosyltransferaseProceedings of the National Academy of Sciences, 1971
- In vitrol culture ofDrosophila melanogaster embryonic cellsIn Vitro Cellular & Developmental Biology - Plant, 1970
- Selection of Hybrids from Matings of Fibroblasts in vitro and Their Presumed RecombinantsScience, 1964