Mapping of brain areas expressing RNA homologous to two different acetylcholine receptor alpha-subunit cDNAs.
- 1 June 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (11), 4076-4080
- https://doi.org/10.1073/pnas.83.11.4076
Abstract
We have used an in situ RNA.cntdot.RNA hybridization technique to determine, in the central nervous systems of the mouse and rat, the distribution of RNA homologous to cDNA clones encoding the .alpha. subunit of a putative neural nicotinic acetylcholine receptor and the .alpha. subunit of the muscle nicotinic acetylcholine receptor. Hybridization of the neural .alpha.-subunit probe was strongest in the medial habenula but was also detected consistently in the compact part of the substantia nigra and ventral tegmental area, in the neocortex, and in certain parts of the thalamus and hypothalamus. The in situ hybridization technique makes it possible to compile a map of brain regions containing cell bodies expressing RNA coding for a specific receptor type and subsequently to apply the techniques of molecular biology to study these brain receptors.Keywords
This publication has 33 references indexed in Scilit:
- Isolation of a cDNA clone coding for a possible neural nicotinic acetylcholine receptor α-subunitNature, 1986
- Denervation supersensitivity in skeletal muscle: analysis with a cloned cDNA probe.The Journal of cell biology, 1984
- Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probesDevelopmental Biology, 1984
- Organization of Ovine Corticotropin-Releasing Factor Immunoreactive Cells and Fibers in the Rat Brain: An Immunohistochemical StudyNeuroendocrinology, 1983
- Stereospecific Nicotine Receptors on Rat Brain MembranesScience, 1980
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- Regulation of dopamine release by presynaptic nicotinic receptors in rat striatal slices: Effect of nicotine in a low concentrationLife Sciences, 1979
- Efferent connections of the habenular nuclei in the ratJournal of Comparative Neurology, 1979
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Afferent connections of the habenular nuclei in the rat. A horseradish peroxidase study, with a note on the fiber‐of‐passage problemJournal of Comparative Neurology, 1977