MALDI‐MS for C‐terminal sequence determination of peptides and proteins degraded by carboxypeptidase Y and P
- 2 January 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 357 (1), 65-69
- https://doi.org/10.1016/0014-5793(94)01323-s
Abstract
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been used for C-terminal amino acid sequence determination of peptides and proteins. The usefulness of MALDI-MS was demonstrated by analyzing peptide mixtures (C-terminal peptide ladder) which were generated by enzymatic digestion of substance P, glucagon, angiotensinogen, insulin B chain and myoglobin with the exopeptidases carboxypeptidase Y and P. The results clearly show that up to 11 amino acid residues can be determined in the pmol range by analyzing the molecular masses of the truncated peptides. For proteins it is possible to investigate enzymatic or chemical digests in the same manner.Keywords
This publication has 22 references indexed in Scilit:
- Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry of Chemically Modified OligonucleotidesAnalytical Chemistry, 1994
- Time-of-Flight Mass Spectrometry Of Underivatized Single-Stranded DNA Oligomers by Matrix-Assisted Laser DesorptionAnalytical Chemistry, 1994
- Protein Ladder SequencingScience, 1993
- Strategy for the characterization of the glycoprotein endoglucanase I isolated from Trichoderma reesei: combination of plasma desorption and UV laser desorption time-of-flight mass spectrometryAnalytica Chimica Acta, 1990
- Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltonsAnalytical Chemistry, 1988
- Use of immobilized exopeptidases and volatile buffers for analysis of peptides by fast atom bombardment mass spectrometryJournal of Mass Spectrometry, 1987
- On-line peptide sequencing by enzymic hydrolysis, high performance liquid chromatography, and thermospray mass spectrometryJournal of the American Chemical Society, 1984
- The combined use of enzymatic hydrolysis and fast atom bombardment mass spectrometry for peptide sequencingJournal of Mass Spectrometry, 1983
- Peptide sequencing using the combination of Edman degradation, carboxypeptidase digestion and Fast Atom Bombardment mass spectrometryBiochemical and Biophysical Research Communications, 1982