hKCNMB3 and hKCNMB4, cloning and characterization of two members of the large‐conductance calcium‐activated potassium channel β subunit family
Top Cited Papers
- 23 May 2000
- journal article
- Published by Wiley in FEBS Letters
- Vol. 474 (1), 99-106
- https://doi.org/10.1016/s0014-5793(00)01584-2
Abstract
We cloned two β subunits of large-conductance calcium-activated potassium (BK) channels, hKCNMB3 (BKβ1) and hKCNMB4 (BKβ4). Profiling mRNA expression showed that hKCNMB3 expression is enriched in testis and hKCNMB4 expression is very prominent in brain. We coexpressed BK channel α (BKα) and BKβ4 subunits in vitro in CHO cells. We compared BKα/β4 mediated currents with those of smooth muscle BKα/β1 channels. BKβ4 slowed activation kinetics more significantly, led to a steeper apparent calcium sensitivity, and shifted the voltage range of BK current activation to more negative potentials than BKβ1. BKα/β4 channels were not blocked by 100 nM charybdotoxin or iberiotoxin, and were activated by 17β-estradiol.Keywords
This publication has 39 references indexed in Scilit:
- Cloning and Functional Characterization of Novel Large Conductance Calcium-activated Potassium Channel β Subunits, hKCNMB3 and hKCNMB4Journal of Biological Chemistry, 2000
- High-Conductance Calcium-Activated Potassium Channels in Rat Brain: Pharmacology, Distribution, and Subunit CompositionBiochemistry, 1999
- Molecular basis of fast inactivation in voltage and Ca 2+ -activated K + channels: A transmembrane β-subunit homologProceedings of the National Academy of Sciences, 1999
- The β Subunit Increases the Ca2+ Sensitivity of Large Conductance Ca2+-activated Potassium Channels by Retaining the Gating in the Bursting StatesThe Journal of general physiology, 1999
- Human and Rodent MaxiK Channel β-Subunit Genes: Cloning and CharacterizationGenomics, 1999
- The β Subunit of the High Conductance Calcium-activated Potassium ChannelJournal of Biological Chemistry, 1998
- Identification of Ca 2+ –activated K + channel splice variants and their distribution in the turtle cochleaProceedings Of The Royal Society B-Biological Sciences, 1998
- Molecular constituents of maxi KCa channels in human coronary smooth muscle: predominant α+β subunit complexesThe Journal of Physiology, 1997
- A calcium switch for the functional coupling between α (hslo) and β subunits (KV, Caβ) of maxi K channelsFEBS Letters, 1996
- Calcium-activated potassium channels expressed from cloned complementary DNAsNeuron, 1992