Toxin Production by Clostridium parabotulinum Type A

Abstract

Factors which govern toxin production by Clostridium parabotulinum type A were studied in growing cultures and in non-proliferating cell suspensions. With growing cultures high toxin titres, comparable to those obtained in complex media, were obtained in a defined medium containing the following essential constituents: arginine, cysteine, histidine, isoleucine, leucine, methionine, phenylalanine, serine (or glycine), tyrosine, tryptophan, valine, glucose, vitamins, phosphate and magnesium. Most of the toxin was formed only after cessation of growth.