Transport of antibiotics and metabolite analogs by systems under cyclic AMP control: positive selection of Salmonella typhimurium cya and crp mutants
- 1 January 1978
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 133 (1), 149-157
- https://doi.org/10.1128/jb.133.1.149-157.1978
Abstract
Mutants in the cyclic(c)AMP control system in S. typhimurium (cya = adenyl cyclase, crp = cAMP receptor protein) were partially resistant to growth inhibition by 22 antibiotics (including fosfomycin, nalidixic acid and streptomycin) and 29 inhibitory analogs of normal bacterial fuel/C sources. This resistance was used as the basis of an efficient positive selection of cya and crp mutants. These antibiotics and analogs enter the bacteria through transport systems normally used for transporting fuel/C sources and this is accomplished because of a structural similarity between the antibiotic and the natural substrate of the particular transport system involved. These transport systems are all under positive control by cAMP and cAMP acts as a signal molecule (alarmone) for fuel/C deprivation. Evidence is provided for a hierarchy within operons controlled by cAMP. The methodology is useful for analyzing both antibiotic transport systems and the cAMP super-control system.This publication has 52 references indexed in Scilit:
- On the Transport of Tripeptide Antibiotics in BacteriaEuropean Journal of Biochemistry, 1976
- The Selectivity of TranscriptionAnnual Review of Biochemistry, 1974
- THE MECHANISM OF ACTION OF FOSFOMYCIN (PHOSPHONOMYCIN)Annals of the New York Academy of Sciences, 1974
- Cyclic Adenosine Monophosphate in BacteriaScience, 1970
- Isolation of UGA and UAG nonsense mutants of bacteriophage P22Virology, 1970
- Pleiotropic deficiency of carbohydrate utilization in an adenyl cyclase deficient mutant of Escherichia coliBiochemical and Biophysical Research Communications, 1969
- .alpha.-Hydrazino analog of histidineJournal of Medicinal Chemistry, 1968
- Genetic control of the l-α-glycerophosphate system in Escherichia coliJournal of Molecular Biology, 1968
- Frameshift Mutagenesis in SalmonellaCold Spring Harbor Symposia on Quantitative Biology, 1966
- Uptake of amino acids by Salmonella typhimuriumArchives of Biochemistry and Biophysics, 1964