Cloning and expression of human brain type I inositol 1,4,5‐trisphosphate 5‐phosphatase High levels of mRNA in cerebellar Purkinje cells
- 20 June 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 347 (1), 69-72
- https://doi.org/10.1016/0014-5793(94)00509-5
Abstract
In brain and many other tissues, Type I inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase is the major isozyme hydrolysing the calcium-mobilizing second messenger InsP3. We recently reported the cloning and expression of dog thyroid InsP3 5-phosphatase. During the course of this cloning, screening of a human brain cDNA library allowed us to isolate a cDNA clone D1 with 91% sequence identity with the thyroid sequence. When clone D1 was expressed in Escherichia coli, the fusion protein had InsP3 5-phosphatase activity. M r estimates of the recombinant enzyme made by unmunodetection, activity assay after SDS/PAGE or silver staining were consistent with the calculated molecular mass. In situ hybridization on human cerebellum sections localised the mRNA for this enzyme to the Purkinje cells.Keywords
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