Cell Cycle Distribution of Proliferative and Functional Cells of the Rat Jejunum after Treatment with Oral E2Prostaglandins

Abstract

Proliferative and functional epithelial cells were isolated from jejunal specimens of the rat by means of vibrational treatment combined with differential air insufflation. This method gave a good separation between superficial cells of the villi and the crypt cells, as evaluated by flow cytometry, morphology, cytology, and incorporation of radioactive thymidine into DNA. Groups of Sprague-Dawley rats (260 g) were treated twice daily for 11 days with oral placebo, 15(R)15-methyl-prostaglandin E2 in the range of 0.125-2 mg .cntdot. kg-1, or 5 mg .cntdot. kg-1 natural PGE2. Isolated crypt cells and superficial cells of the jejunal villi were then analysed by flow cytometry. Morphometric measurements were performed on sections of some jejunal specimens not submitted to vibrational treatment. The cell cycle distribution of crypt cells was unaffected by treatment with the prostaglandin analogue despite the presence of trophic changes. The proportion of crypt cells in G2/M phase was slightly but significantly reduced in rats given natural PGE2 compared with controls. The cell cycle distribution of villus cells was not affected by prostaglandin treatment. Trophic changes in the absence of increased DNA synthesis (S phase) or increased mitotic activity suggests that the hyperplasia observed after prostaglandin treatment is due to a reduced cell loss and/or slower migration time of epithelial cells.