Fine Specificity and Cytolytic Activity of Continuously Growing Alloreactive Cytotoxic T Lymphocyte Clones

Abstract

The role of Lyt I+ T[thymus-derived]-cell-derived secondary CTL [cytotoxic T lymphocyte]-inducing factor allowed the cloning of alloreactive [mouse CTL] by the limiting dilution approach. Several monoclonal cell lines were established in vitro. The lytic activity of some of the cell lines exceeded that of CTL from bulk cultures; 50% of the target cells were lysed at an effector to target cell ratio of 0.04:1. The fine specificity of individual CTL clones is discussed.