C‐type natriuretic peptide in bovine chromaffin cells The regulation of its biosynthesis and secretion

Abstract

We report here the regulation of the biosynthesis and the secretion of C-type natriuretic peptide (CNP) in cultured bovine chromaffin cells. The combined treatment with protein kinase A and -C activators induced a 6-fold increase of intracellular levels of CNP-(1–103). The biosynthesized CNP-(1–103) was co-released with its mature forms, typically CNP-(51–103), upon stimulation by nicotine or depolarizing agents. This confirms the neuropeptidic character of this third member of the natriuretic peptide family, which might act as a neuromodulator or neurotransmitter