An experimental study of the heat transfer distribution in a turbine rotor passage was conducted in a large-scale, ambient temperature, rotating turbine model. Heat transfer was measured for both the full-span suction and pressure surfaces of the airfoil and for the hub endwall surface. The objective of this program was to document the effects of flow three dimensionality on the heat transfer in a rotating blade row (versus a stationary cascade). Of particular interest were the effects of the hub and tip secondary flows, tip leakage, and the leading-edge horseshoe vortex system. The effect of surface roughness on the passage heat transfer was also investigated. Midspan results are compared with both smooth-wall and rough-wall finite-difference two-dimensional heat transfer predictions. Contour maps of Stanton number for both the rotor airfoil and endwall surfaces revealed numerous regions of high heat transfer produced by the three-dimensional flows within the rotor passage. Of particular importance are regions of local enhancement (as much as 100 percent over midspan values) produced on the airfoil suction surface by the secondary flows and tip-leakage vortices and on the hub endwall by the leading edge horseshoe vortex system.