Recombinants between herpes simplex virus types 1 and 2: analyses of genome structures and expression of immediate early polypeptides
- 1 November 1978
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 28 (2), 499-517
- https://doi.org/10.1128/jvi.28.2.499-517.1978
Abstract
Recombinants between temperature-sensitive mutants of herpes simplex virus types 1 (HSV-1) and 2 (HSV-2) were constructed. Using restriction endonucleases, the genome composition of 17 intertypic recombinants was analyzed and crossovers in every region of the genome were detected. The virion DNA of 1 recombinant appeared to be largely frozen in 2 of the 4 possible genome arrangements of HSV. Knowledge of the genome structures of recombinants enabled physical mapping of immediate early polypeptides. The immediate early polypeptide Vmw IE 110 of HSV-1 and its functionally equivalent polypeptide, Vmw IE 118, of HSV-2 may map in the repetitive sequences bounding the long unique region of HSV.This publication has 36 references indexed in Scilit:
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