Synaptic corelease of ATP and GABA in cultured spinal neurons
- 1 March 1999
- journal article
- research article
- Published by Springer Nature in Nature Neuroscience
- Vol. 2 (3), 241-245
- https://doi.org/10.1038/6344
Abstract
In the spinal dorsal horn (DH), transmission and modulation of peripheral nociceptive (pain-inducing) messages involve classical neurotransmitters and neuropeptides. We show that approximately half of DH neurons use ATP as a fast excitatory neurotransmitter acting at ionotropic P2X postsynaptic receptors. ATP was not codetected with glutamate but was coreleased with the inhibitory neurotransmitter GABA. Moreover, adenosine, probably generated by extracellular metabolism of ATP, finely tuned GABAergic inhibitory postsynaptic currents. Differential modulation of excitatory versus inhibitory components of this mixed cotransmission may help to explain changes in sensory message processing in the DH during mechanical hyperalgesia and neuropathic pain.Keywords
This publication has 38 references indexed in Scilit:
- Oxytocin Modulates Glutamatergic Synaptic Transmission between Cultured Neonatal Spinal Cord Dorsal Horn NeuronsJournal of Neuroscience, 1998
- Electrophysiological properties of cultured neonatal rat dorsal horn neurons containing GABA and met-enkephalin-like immunoreactivity.Journal of Neurophysiology, 1998
- ATP P2XReceptors Mediate Fast Synaptic Transmission in the Dorsal Horn of the Rat Spinal CordJournal of Neuroscience, 1997
- Purinergic receptors: their role in nociception and primary afferent neurotransmissionCurrent Opinion in Neurobiology, 1996
- Cloning OF P2X5 and P2X6 receptors and the distribution and properties of an extended family of ATP-gated ion channelsJournal of Neuroscience, 1996
- The localization of classical transmitters and neuropeptides within neurons in laminae I–III of the mammalian spinal dorsal hornProgress in Neurobiology, 1993
- Physiological roles for adenosine and ATP in synaptic transmission in the spinal dorsal hornProgress in Neurobiology, 1993
- ATP release from dorsal spinal cord synaptosomes: characterization and neuronal originBrain Research, 1993
- Primary afferent-evoked synaptic responses and slow potential generation in rat substantia gelatinosa neurons in vitroJournal of Neurophysiology, 1989
- Is ATP a central synaptic mediator for certain primary afferent fibers from mammalian skin?Proceedings of the National Academy of Sciences, 1984