Isolation and characterization of membrane proteins responsible for attachment of polyribosomes to rough microsomal fraction of rat liver
- 15 April 1977
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 164 (1), 53-66
- https://doi.org/10.1042/bj1640053
Abstract
A protein fraction which has a high affinity for polyribosomes was isolated from rough microsomal membranes of rat liver. The mode of polyribosome binding to this fraction (R-fraction) was studied by using CsCl equilibrium centrifugation and compared with that for stripped rough microsomal membranes. The polyribosome-binding capacity of the R-fraction was heat-labile and sensitive to trypsin, and was suppressed by increasing KCl concentration and addition of 0.1 mM-aurintricarboxylic acid. Of the 4 subfractions obtained by gel filtration of the R-fraction on a Sephadex G-200, only the R1-fraction, eluted at the void volume, showed a high affinity for polyribosomes. The polyribosome-binding capacity of the R1-fraction decreased with time on storage at 4.degree. C. The R1-fraction contained 3 major proteins with MW 108,000, 99,000 and 65,000.This publication has 34 references indexed in Scilit:
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