• 1 January 1980
    • journal article
    • research article
    • Vol. 4 (5-6), 39-46
Abstract
A short-term initiation/promotion assay in rat liver for chemical carcinogens was studied. Diethylnitrosamine was used as the initiator and phenobarbital and partial hepatectomy as promoters. Initiation was determined as the focal induction of .gamma.-glutamyl transpeptidase activity. The protocol of the assay consisted of the following: the initiator was administered; 1 wk later the rats were given 500 ppm phenobarbital in their drinking water; 1 wk later 2/3 partial hepatectomy was performed and the rats were maintained on 100 ppm phenobarbital for an additional 4 wks.