Tumor Induction in Germfree Rats With Methylazoxymethanol (MAM) and Synthetic MAM Acetate2
- 1 August 1967
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 39 (2), 355-371
- https://doi.org/10.1093/jnci/39.2.355
Abstract
The glucoside cycasin, an effective hepatotoxin and carcinogen in conventional rats, fails to produce these effects when administered to germfree rats. The hepatotoxic and carcinogenic effects of cycasin can also be elicited after prior hydrolysis to the aglycone. The aglycone (MAM) and the synthetic aglycone acetate ester produce all the effects in germfree rats of which the intact glucoside is capable only when fed to conventional rats. The aglycone is therefore the proximate carcinogen. Its liberation from the glucoside in conventional rats is mediated in the intestinal tract by a β-glucosidase of bacterial origin. Intraperitoneal administration of the synthetic aglycone acetate and the free aglycone appears to be the most effective route for tumor induction and, of these resulting tumors, the most frequent are in the intestinal tract.Keywords
This publication has 4 references indexed in Scilit:
- Role of Intestinal Microorganisms in Determining Cycasin ToxicityExperimental Biology and Medicine, 1967
- Studies on methylazoxymethanol, the aglycone of cycasinArchives of Biochemistry and Biophysics, 1965
- The induction of intestinal neoplasms in rats with the glycoside cycasin and its aglyconeVirchows Archiv, 1965
- Carcinogenic Properties of Nuts From Cycas Circinalis L. Indigenous to Guam2JNCI Journal of the National Cancer Institute, 1963