Nucleotide sequence of wheat germ cytoplasmic initiator methionine transfer ribonucleic acid
Open Access
- 25 May 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (10), 3241-3247
- https://doi.org/10.1093/nar/10.10.3241
Abstract
The primary sequence of wheat germ initiator tRNA has been determined using in vitro labelling techniques. The sequence is: pAUCAGAGUm1Gm2GCGCAG CGGAAGCGUm2GGψGGGCCCAUt6AACCCACAGm7GDm5Cm5CCAGGAψCGm1AAACCUG*GCUCUGAUACCAOH−. As in other eukaryotic initiator tRNAs, the sequence -TψCG(A)- present in loop IV of virtually all tRNA active in protein synthesis is absent and is replaced by -AψCG-. The base pair G2:C71 present in all other initiator tRNAs recognized by E. coli Met-tRNA transformylase is absent and is replaced by U2:A71. Since wheat germ initiator tRNA is not formylated by K. coli Met-tRNA transformylase this implies a possible role of the G2:C71 base pair present in other initiator tRNAs in formylatlon of initiator tRNA species.Keywords
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