An inducible translocation strategy to rapidly activate and inhibit small GTPase signaling pathways
- 20 May 2005
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 2 (6), 415-418
- https://doi.org/10.1038/nmeth763
Abstract
We made substantial advances in the implementation of a rapamycin-triggered heterodimerization strategy. Using molecular engineering of different targeting and enzymatic fusion constructs and a new rapamycin analog, Rho GTPases were directly activated or inactivated on a timescale of seconds, which was followed by pronounced cell morphological changes. As signaling processes often occur within minutes, such rapid perturbations provide a powerful tool to investigate the role, selectivity and timing of Rho GTPase–mediated signaling processes.Keywords
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