Human Factor VIII: Morphometric Analysis of Purified Material in Solution
- 2 December 1977
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 198 (4320), 932-934
- https://doi.org/10.1126/science.303809
Abstract
Study of purified human factor VIII in buffer by freeze-etch electron microscopy reveals rounded, rod-shaped particles measuring 22 by 42 nanometers. When thrombin was added to purified normal factor VIII, there was a rapid loss of rod-shaped particles during the first 15 minutes of incubation at 37 degrees C. Purified plasma from two patients with severe hemophilia contained spherical particles measuring 10 to 50 nanometers in diameter, with no evidence of significant numbers of rod-shaped forms. Negatively stained and unstained air-dried samples of factor VIII corroborate the relative shape and size differences between normal and hemophiliac material.This publication has 7 references indexed in Scilit:
- Studies on human antihemophilic factor. Evidence for a covalently linked subunit structure.JCI Insight, 1976
- The Subunit Structure of Normal and Hemophilic Factor VIIIJCI Insight, 1973
- Studies on the purification and characterization of human factor VIIIJCI Insight, 1972
- Electron microscopy of tobacco mosaic virus under conditions of minimal beam exposureJournal of Molecular Biology, 1970
- Studies on the purification of antihemophilic factor (factor VIII)JCI Insight, 1969
- Studies on the purification of antihemophilic factor (factor VIII)JCI Insight, 1969