Release of Monoamines from Striatum of Rat and Mouse Evoked by Local Application of Potassium: Evaluation of a New In Vivo Electrochemical Technique
- 1 March 1986
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 46 (3), 842-850
- https://doi.org/10.1111/j.1471-4159.1986.tb13048.x
Abstract
Local application of K+ via micropressure-ejection, coupled with in vivo electrochemical detection, was used to study stimulated release from monoaminergic nerve terminals in the striatum of anesthetized rats and mice. K − -evoked releases were reversible, reproducible, and dose-dependent. In contrast, releases of electroactive species could not be evoked by local ejection of Na+. The magnitudes and time courses of K+-evoked releases recorded from the caudate nucleus of mice were greater than those seen in rats. Local application of nomifensine, a putative catecholamine reuptake blocker, augmented the magnitudes and time courses of K+-evoked releases. Releases were also recorded from brain regions adjacentthe striatum; these signals were always smaller than those seen in the caudate nucleus and had amplitudes that showed good correspondence to the relative degree of dopaminergic input to these areas. These data, taken together with other information in the literature, suggest that this new technique is well suited for in situ studies of monoamine release and reuptake in intact animals.Keywords
This publication has 41 references indexed in Scilit:
- Fast in vivo monitoring of dopamine release in the rat brain with differential pulse amperometryAnalytical Chemistry, 1985
- Direct in Vivo Monitoring of Dopamine Released from Two Striatal Compartments in the RatScience, 1983
- Direct measurement of the effect of potassium, calcium, veratridine, and and amphetamine on the rate of release of dopamine from superfused brain tissueLife Sciences, 1981
- Microprocessor-controlled apparatus for in vivo electrochemical measurementAnalytical Chemistry, 1980
- Physical and physiological characteristics of micropressure ejection of drugs from multibarreled pipettesNeuropharmacology, 1980
- Release of endogenous norepinephrine and dopamine from rat brain regions in vitroLife Sciences, 1978
- Release of endogenous dopamine from corpus striatumLife Sciences, 1978
- 243 - Brain dopaminergic neurons: In vivo electrochemical information concerning storage, metabolism and release processesBioelectrochemistry and Bioenergetics, 1978
- Calciumm-dependent release of endogenous serotonin, dopamine and norepinephrine from nerve endingsLife Sciences, 1977