MODIFICATION OF THE PANCREATIC RESPONSE TO SECRETIN BY URINE AND URINE CONCENTRATES

Abstract

Normal human urine has the property of inactivating secretin incubated with it. This property is lost when the urine is boiled prior to incubation, indicating that the inactivation is on an enzymic basis and that urine contains a Secretinase which may or may not be identical with the secretinase previously demonstrated to be present in the blood. Various urine concentrates were prepared in an attempt to secure more potent enzyme preps.; and the inj. of these depressed the secretory activity of the pancreas in response to secretin for 1-2 hrs. This effect was obtained with both boiled and unboiled concentrates. Thus urine contains the secretin-inactivating enzyme secretinase, and also a heat-stable substance, uropancreatone, which acts directly on the pancreas to inhibit its secretion.