FLUOROMETRIC QUANTITATION OF FLUORESCEIN-COUPLED ANTIBODIES ATTACHED TO THE CELL MEMBRANE
- 1 August 1969
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 63 (4), 1157-1163
- https://doi.org/10.1073/pnas.63.4.1157
Abstract
A method was devised which allows the measurement, by direct and indirect fluorescence assay, of the binding of fluorescein-coupled antibodies directed against cell-membrane antigens. The systems used were: (1) species-specific antigens, (2) genetically determined isoantigens, (3) virus-determined, tumor-specific antigens, and (4) membrane-bound immunoglobulin in a Burkitt cell line. Titration of antibodies gave straight lines in the log versus log scale, the slope of the titration curve probably being dependent on the density of antigen receptors on the cell surface.Keywords
This publication has 7 references indexed in Scilit:
- Studies on murine sarcoma virus: antigenic characterization of murine sarcoma virus induced tumor cellsInternational Journal of Cancer, 1969
- Direct membrane immunofluorescence reaction of Burkitt's lymphoma cells in culture.1969
- Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines.1968
- Fluorometric estimation of antigens (antibodies)Zeitschrift für Naturforschung B, 1965
- CHEMICAL AND PHYSICAL VARIABLES AFFECTING FLUORESCEIN ISOTHIOCYANATE AND ITS PROTEIN CONJUGATESPublished by Defense Technical Information Center (DTIC) ,1965
- A REVISED NOMENCLATURE FOR THE HISTOCOMPATIBILITY-2 LOCUS OF THE MOUSETransplantation, 1964
- Antigenic Properties of Lymphomas Induced by the Moloney Agent2JNCI Journal of the National Cancer Institute, 1964