CHROMOSOME-SPECIFIC ALPHA SATELLITE DNA FROM THE CENTROMERE OF HUMAN CHROMOSOME-16
- 1 December 1989
- journal article
- research article
- Vol. 45 (6), 862-872
Abstract
To examine the molecular organization of DNA sequences located in the centromeric region of human chromosome 16 we have isolated and characterized a chromosome 16-specific member of the alpha satellite DNA family. The probe obtained is specific for the centromere of chromosome 16 by somatic cell hybrid analysis and by fluorescence in situ hybridization and allows detection of specific hybridizing domains in interphase nuclei. Nucleotide sequence analysis indicates that this class of chromosome 16 alpha satellite (D16Z2) is organized as a series of diverged 340-bp dimers arranged in a tandem array of 1.7-kb higher-order repeat units. As measured by pulsed-field gel electrophoresis, the total D16Z2 array spans .apprx. 1,400-2,000 kb of centrophoresis and by pulsed-field gel electrophoresis. Investigation of this family of alpha satellite should facilitate the further genomic. cytogenetic, and genetic analysis of chromosome 16.This publication has 36 references indexed in Scilit:
- Chromosome-specific alpha satellite DNA: Isolation and mapping of a polymorphic alphoid repeat from human chromosome 10Genomics, 1988
- Rapid interphase and metaphase assessment of specific chromosomal changes in neuroectodermal tumor cells by in situ hybridization with chemically modified DNA probesExperimental Cell Research, 1988
- Chromosome-specific alpha satellite DNA from human chromosome 1: Hierarchical structure and genomic organization of a polymorphic domain spanning several hundred kilobase pairs of centromeric DNAGenomics, 1987
- Structure of the major block of alphoid satellite DNA on the human Y chromosomeJournal of Molecular Biology, 1987
- Chromosome-specific subfamilies within human alphoid repetitive DNAJournal of Molecular Biology, 1986
- Identification of a human clustered G + C-rich DNA family of repeats (Sau3A family)Journal of Molecular Biology, 1985
- The Origin of Man: A Chromosomal Pictorial LegacyScience, 1982
- Repeated sequence specific to human malesNature, 1976
- Human Y-Chromosome-Specific Reiterated DNAScience, 1976
- Repeated sequences in human DNAJournal of Molecular Biology, 1970