Pressure Accelerated Chromatography Through Microparticulate Silica Columns Packed by Centrifugation

Abstract

Centrifugal microparticulate gel chromatography, in which centrifugal force is used to pack the adsorbent bed and to accelerate development, has been modified to permit sample components to be separated, eluted, monitored, and collected. This modification also allows the size of the sample to be significantly increased. The bed is packed by centrifugal force as before, but the developing solvent is forced through the column by applying modest hydrostatic pressures. This modified technique has been used to isolate: 1) microgram quantities of dapsone metabolites, 2) microgram quantities of mono- and disaccharides, and 3) milligram amounts of chromatographically pure, immunologically active components from a complex mycobacterial wax.