Determination of Plasma Fibrinogen by Means of Centrifugation After Heating

Abstract

The paper deals with a relatively simple and rapid micromethod for the quantitative determination of fibrinogen. It is based on the precipitation of fibrinogen in oxalated plasma in microhematocrit tubes, by means of heating at 56 C for 15 min., following which the tubes are centrifuged at approximately 11,000 r.p.m. for 2 min. The height of the fibrinogen precipitate is read by means of a microscale using an inverted eyepiece of a standard microscope. The scale reading is standardized by the Kjeldahl method. The method is not accurate in the presence of jaundice.