Selection of reference genes for gene expression studies in pig tissues using SYBR green qPCR
Open Access
- 15 August 2007
- journal article
- Published by Springer Nature in BMC Molecular Biology
- Vol. 8 (1), 67
- https://doi.org/10.1186/1471-2199-8-67
Abstract
Real-time quantitative PCR (qPCR) is a method for rapid and reliable quantification of mRNA transcription. Internal standards such as reference genes are used to normalise mRNA levels between different samples for an exact comparison of mRNA transcription level. Selection of high quality reference genes is of crucial importance for the interpretation of data generated by real-time qPCR.Keywords
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