Phenylalanine and tyrosine in serum and eluates from dried blood spots as determined by reversed-phase liquid chromatography.
Open Access
- 1 July 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 33 (7), 1152-1154
- https://doi.org/10.1093/clinchem/33.7.1152
Abstract
We have developed a reversed-phase liquid-chromatographic procedure for simultaneously determining phenylalanine and tyrosine in serum and eluates of dried blood spots. Batch derivatization with phenylisothiocyanate and a 10-min linear gradient chromatographic assay with ultraviolet absorbance detection provide rapid sample throughput. Interrun precision (CV) is less than 12%; analytical recovery (from blood spot samples) exceeds 85%. Results for patients' samples correlate well with those from an amino acid analyzer and we encountered no apparent interferences. The speed and specificity of this assay facilitate the rapid diagnosis and monitoring of patients with phenylketonuria.This publication has 3 references indexed in Scilit:
- Comparative column chromatographic estimations of phenylalanine in plasma, whole blood, native and paper‐dried capillary blood of healthy children and adults, and patients with hyperphenylalaninaemiaJournal of Inherited Metabolic Disease, 1984
- Dansylation of Amino Acids and Byproduct FormationJournal of Liquid Chromatography, 1984
- REPORT ON A COOPERATIVE STUDY OF VARIOUS FLUOROMETRIC PROCEDURES AND GUTHRIE BACTERIAL INHIBITION ASSAY IN DETERMINATION OF HYPERPHENYLALANINEMIA1973