Extraction and reverse‐phase high‐pressure liquid chromatography of gibberellic acid in soil
- 1 April 1988
- journal article
- research article
- Published by Taylor & Francis in Communications in Soil Science and Plant Analysis
- Vol. 19 (5), 617-624
- https://doi.org/10.1080/00103628809367962
Abstract
Several aqueous extractants buffered above pH 6.5 were evaluated for their ability to extract gibberellic acid from a Xerollic Calciorthid. More than 90% of the GA3 added to soil was recovered with 0.01 M KH2PO4, pH 7.4. Gibberellic acid in soil extracts concentrated under vacuum was determined by reverse‐phase high‐pressure liquid chromatography (HPLC) by comparing the reproducible GA3 peak areas with those of standards prepared in the same background solution.Keywords
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