Membrane currents in the rabbit sinoatrial node cell as studied by the double microelectrode method

Abstract

When a strand of the rabbit sinoatrial node tissue was shortened by ligation, the spatial decay of electrotonic potential decreased and the input impedance increased. In a piece of the tissue 0.2–0.3 mm in diameter apparently uniform current spread was obtained. Action potentials recorded from three different sites in this small piece occurred simultaneously and were superimposable. In voltage clamp experiments using the double microelectrode method, the membrane potential was usually held at −30 to −40 mV, where no net current flowed. When membrane potential was suddenly changed from the holding potential, the sign and the time course of the ionic current varied with membrane potential. Hyperpolarization gave an inward current which increased with time. Depolarization gave a transient inward current followed by sustained outward current, and repolarization gave an outward current tail which exponentially subsided with a time constant of 0.37 s. The membrane time constant was 12.0 ms. When the specific membrane capacitance was assumed to be 1 μF/cm², the specific membrane resistance at the resting potential was 12 kΩcm². The peak of the transient inward current on depolarization was 1.3×10⁻⁵ A/cm².