Immunochemical Studies of Partially Hydrolyzed Lipopolysaccharide from Fusobacterium nucleatum Fev1

Abstract

F. nucleatum Fev1 lippoplysaccharide was split by hydrolysis with 1% acetic acid into acid-soluble polysaccharide and lipid A. Gel filtration of the polysaccharide on Bio-Gel P-60 gave a high-molecular-weight fraction eluted with the void volume, and a fraction eluted at 2.4 .times. Vo. The high MW fraction contained L-glycero-D-manno-heptose in relative large amounts, glucose, glucosamine, an unknown amino compound and small amounts of (or no) D-glycero-D-manno-heptose. Phosphorus and 3-deoxy-D-manno-octulosonic acid were not detected. The other fraction contained L- and D-glycero-D-manno-heptose, glucose, glucosamine, 3-deoxy-D-manno-octulosonic acid and phosphorus. Further fractionation experiments and serological investigations indicated that the high-MW fraction carried the O-antigenic side chains, but the material eluted for Bio-Gel P-60 at 2.4 .times. Vo represented the core oligosaccharide.