Interactions of fibronectin with Treponema pallidum.
- 1 June 1985
- journal article
- research article
- Published by BMJ in Sexually Transmitted Infections
- Vol. 61 (3), 147-155
- https://doi.org/10.1136/sti.61.3.147
Abstract
The adhesive or opsonic glycoprotein, fibronectin, is associated with the surface of T. pallium as shown by immunofluorescence. A quantitative assay using 125I showed that T. pallidum harvested 7 days after infection bound more fibronectin than T. pallidum harvested 14 days after infection. This increased binding by younger organisms was confirmed by radioimmunoassay techniques. Fibronectin appears to have a role in treponemal attachment. Preincubation of T. pallidum with goat or rabbit antibody to fibronectin blocked treponemal attachment to cultured cells and to isolated capillaries and inhibited treponemal virulence. Treponemes were incubated in glass wool columns pretreated with fibronectin and were then eluted from the columns. This technique yielded a population of T. pallidum that failed to bind to fibronectin. Compared with treponemes eluted form control ovalbumin columns, organisms eluted from fibronectin columns attached to cultured cells in larger numbers but did not survive as long and were not as virulent. Findings are discussed in terms of the relevance of interaction between treponemes and fibronectin in the pathogenesis of T. pallidum.This publication has 56 references indexed in Scilit:
- Binding of glycosaminoglycans to the surface of Treponema pallidum and subsequent effects on complement interactions between antigen and antibody.Sexually Transmitted Infections, 1985
- Attachment of Treponema pallidum to fibronectin, laminin, collagen IV, and collagen I, and blockage of attachment by immune rabbit IgG.Sexually Transmitted Infections, 1984
- Immune-mediated injury to basement membranes in mice immunized with murine lamininClinical Immunology and Immunopathology, 1984
- Fibronectin Binds to Some Bacteria But Does Not Promote Their Uptake by Phagocytic CellsScience, 1983
- Interaction of Treponema pallidum with isolated rabbit capillary tissues.Sexually Transmitted Infections, 1983
- Binding of fibronectin to the surface of group A, C, and G streptococci isolated from human infectionsEuropean Journal of Clinical Microbiology & Infectious Diseases, 1982
- Antibodies to laminin in Chagas' disease.The Journal of Experimental Medicine, 1982
- CLUMPING OF STAPHYLOCOCCUS AUREUS BY HUMAN FIBRONECTINActa Pathologica Microbiologica Scandinavica Section B Microbiology, 1981
- The Humoral Immune Response in Rabbits Infected with Treponema pallidumSexually Transmitted Diseases, 1980
- Molecular characterization of receptor binding proteins and immunogens of virulent Treponema pallidum.The Journal of Experimental Medicine, 1980