Molecular structure of (m5dC-dG)3: the role of the methyl group on 5-methyl cytosine in stabilizing Z-DNA
Open Access
- 11 December 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (23), 7879-7892
- https://doi.org/10.1093/nar/10.23.7879
Abstract
The hexamer (m 5 dC-dG) 3 has been synthesized and its three-dimensional structure determined by a single crystal X-ray diffraction analysis. The structure has been refined to a final R valne of 15.6% at 1.3 Å resolution. The molecule forms a left-handed Z-DNA helix which is similar to the unmethylated Z-DNA structure. The presence of the methyl group has resulted in slight changes in the twist angle between snccessive base pairs and modification of some of the interatomic contacts. Methylation of cytosine in the C5 position is associated with a relative destabilization of the B-DNA structure and a stabilization through hydrophobic bonding of the Z-DNA structure.Keywords
This publication has 11 references indexed in Scilit:
- Conformation and dynamics in a Z-DNA tetramerJournal of Molecular Biology, 1981
- The high salt form of poly(dG-dC)·poly(dG-dC) is left-handed Z-DNA: Raman spectra of crystals and solutionsNucleic Acids Research, 1981
- 5-Methylcytosine in Eukaryotic DNAScience, 1981
- Effects of methylation on a synthetic polynucleotide: the B--Z transition in poly(dG-m5dC).poly(dG-m5dC).Proceedings of the National Academy of Sciences, 1981
- Left-Handed Double Helical DNA: Variations in the Backbone ConformationScience, 1981
- DNA Methylation and Gene FunctionScience, 1980
- Left-handed DNA helicesNature, 1980
- Molecular structure of a left-handed double helical DNA fragment at atomic resolutionNature, 1979
- Conformational analysis of the sugar ring in nucleosides and nucleotides. New description using the concept of pseudorotationJournal of the American Chemical Society, 1972
- Salt-induced co-operative conformational change of a synthetic DNA: Equilibrium and kinetic studies with poly(dG-dC)Journal of Molecular Biology, 1972