Detection of Low-Copy-Number Genomic DNA Sequences in Individual Bacterial Cells by Using Peptide Nucleic Acid-Assisted Rolling-Circle Amplification and Fluorescence In Situ Hybridization
- 1 April 2007
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 73 (7), 2324-2328
- https://doi.org/10.1128/aem.02038-06
Abstract
An approach is proposed for in situ detection of short signature DNA sequences present in single copies per bacterial genome. The site is locally opened by peptide nucleic acids, and a circular oligonucleotide is assembled. The amplicon generated by rolling circle amplification is detected by hybridization with fluorescently labeled decorator probes.Keywords
This publication has 43 references indexed in Scilit:
- Quantitative Determination of Free-DNA Uptake in River Bacteria at the Single-Cell Level by In Situ Rolling-Circle AmplificationApplied and Environmental Microbiology, 2006
- Microarray-Based Analysis of Subnanogram Quantities of Microbial Community DNAs by Using Whole-Community Genome AmplificationApplied and Environmental Microbiology, 2006
- Environmental Whole-Genome Amplification To Access Microbial Populations in Contaminated SedimentsApplied and Environmental Microbiology, 2006
- Visualization and Enumeration of Bacteria Carrying a Specific Gene Sequence by In Situ Rolling Circle AmplificationApplied and Environmental Microbiology, 2005
- Fluorescencein situhybridisation (FISH) – the next generationFEMS Microbiology Letters, 2005
- Simultaneous Fluorescence In Situ Hybridization of mRNA and rRNA in Environmental BacteriaApplied and Environmental Microbiology, 2004
- Two sides of the coin: affinity and specificity of nucleic acid interactionsTrends in Biochemical Sciences, 2004
- Whole genome amplification: abundant supplies of DNA from precious samples or clinical specimensTrends in Biotechnology, 2003
- In situ detection of a virulence factor mRNA and 16S rRNA inListeria monocytogenesFEMS Microbiology Letters, 1998
- Modern methods in subsurface microbiology: in situ identification of microorganisms with nucleic acid probesFEMS Microbiology Reviews, 1997