Sister chromatid fusion initiates amplification of the dihydrofolate reductase gene in Chinese hamster cells.
Open Access
- 1 April 1993
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 7 (4), 605-620
- https://doi.org/10.1101/gad.7.4.605
Abstract
We have utilized a dihydrofolate reductase (DHFR) probe in combination with selected probes from other positions along the 2q chromosome arm in a two-color fluorescence in situ hybridization analysis of early DHFR gene amplification events in CHO cells. These studies show clearly that the most frequent initiating event is the formation of a giant inverted duplication, resulting from chromosome breakage and terminal fusion or a reverse unequal sister chromatid exchange. The dicentric chromosomes thus formed initiate bridge/breakage/fusion cycles that appear to mediate subsequent amplification steps to higher copy number.Keywords
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