Isolation and Function of the Gene A Initiator of Bacteriophage ϕX 174, A Highly Specific DNA Endonuclease
- 1 April 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (4), 1549-1553
- https://doi.org/10.1073/pnas.71.4.1549
Abstract
The gene A product of Escherichia coli bacteriophage varphiX 174, necessary for initiation of varphiX DNA synthesis, has been identified and purified to near homogeneity. The gene A protein is shown to be a highly specific DNA endonuclease which breaks one phosphodiester bond in the viral strand of either double-stranded, circular varphiX replicative form I (RFI), or in single-stranded, circular varphiX viral DNA; it is inactive against all other species of DNA tested.Keywords
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