Leucocyte Cultures in Five Strains of Mice

Abstract

Leucocytes of five inbred strains of mice (LG, Swiss, SM, C3H and C57) were separated from whole blood by using 6% dextran and then grown in tissue culture medium in the presence of PHA and fetal calf serum. Transformation occurred in all strains, but mitotic divisions were observed only in the LG and Swiss strains at a maximum on the second day of culturing. The maximum mitotic index was 3.4% in both strains. The addition of human AB serum to the cultures increased the death rate of the cells and there was no mitosis.