The Analysis of Mixtures of Closely Related Naturally-occurring Organic Compounds Using High Performance Liquid Chromatography

Abstract

Applications of high performance liquid chromatography involving the analysis of mixtures of closely related organic compounds are described. The compounds used in this study include examples of some of the most important types of naturally occurring organic compounds—flavonoids, peptides, porphyrins, and carbohydrates. Adsorption chromatography is in general the most useful technique for the separation of these compounds, and has been used to separate several different types of structural and stereo-isomers. Results obtained using totally porous and superficially porous packing materials are compared. The use of the solvent strength parameter (#x03B5;^°) as a guide to mobile phase selection is fruitful, and is suggested as a general approach.