Interaction between the Rous sarcoma virus transforming protein and two cellular phosphoproteins: analysis of the turnover and distribution of this complex.
Open Access
- 1 January 1983
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 3 (1), 9-19
- https://doi.org/10.1128/mcb.3.1.9
Abstract
The transforming protein of Rous sarcoma virus (RSV), pp60src, was previously shown to associate with two cellular proteins of Mr 90,000 and 50,000 in RSV-transformed chicken cells. In this report, we demonstrate that this interaction is specific for a discrete population of pp60src molecules. Newly synthesized pp60src was found to preferentially associate with pp90 and pp50 to form a short-lived complex. The half-life of this complex varied from 9 to 15 min in cells transformed by nondefective strains of RSV. This interaction between pp60src, pp50, and pp90 took place in a soluble fraction of the cell, and the complex-bound pp60src molecules were not phosphorylated on tyrosine. These results suggest that pp90 and pp50 may be involved in the processing of pp60src molecules before the association of pp60src with the plasma membrane. The kinetics of dissociation of this complex were shown to be altered in cells infected with viruses containing a temperature-sensitive defect in the src gene. When cells infected with these viruses were grown at the nonpermissive temperature, more than 90% of the pp60src molecules were associated with pp90 and pp50, and little or no dissociation was observed in a 3-h chase period. These results suggest that mutations in the src gene which affect the transforming activity of pp60src also affect the stability of the interaction of pp60src with pp90 and pp50.This publication has 46 references indexed in Scilit:
- Rous sarcoma virus-induced phosphorylation of a 50,000-molecular weight cellular proteinNature, 1982
- The specific interaction of the Rous sarcoma virus transforming protein, pp60src, with two cellular proteinsCell, 1981
- Characterization of protein kinase activity associated with the transforming gene product of Fujinami sarcoma virusCell, 1980
- Identification of a cellular protein substrate phosphorylated by the avian sarcoma virus-transforming gene productCell, 1980
- Transformation by Rous sarcoma virus: A cellular substrate for transformation-specific protein phosphorylation contains phosphotyrosineCell, 1980
- Avian sarcoma virus-transforming protein, pp60src shows protein kinase activity specific for tyrosineNature, 1980
- T antigen is bound to a host protein in SY40-transformed cellsNature, 1979
- The effect of amino acid analogues and heat shock on gene expression in chicken embryo fibroblastsCell, 1978
- Evidence that the transforming gene of avian sarcoma virus encodes a protein kinase associated with a phosphoproteinCell, 1978
- In vitro synthesis of a functional avian sarcoma virus transforming-gene productNature, 1978