Differential reactivity of the red-and far-red-absorbing forms of phytochrome to [14C] N-ethyl maleimide
- 1 January 1974
- journal article
- Published by Springer Nature in Planta
- Vol. 117 (4), 367-372
- https://doi.org/10.1007/bf00388032
Abstract
The red-absorbing form (P r ) and the far-red absorbing form (P fr ) of undergraded, high-molecular-weight phytochrome from rye (Secale cereale L.) seedlings were examined for their reactivity toward N-ethyl-[14C]maleimide ([14C]-NEM). After pre-treatment of P r with cold NEM and extensive dialysis, photoconversion to P fr and treatment with [14C]NEM resulted in an approximately 70% increase in incorporation of radioactivity over the dark control. These results are discussed in relation to the view that phytochrome undergoes a protein conformational change upon phototransformation.Keywords
This publication has 17 references indexed in Scilit:
- SOME PROPERTIES OF PHOTOTRANSFORMATION OF RYE PHYTOCHROME IN VITROPhotochemistry and Photobiology, 1974
- STUDIES ON THE PROTEIN CONFORMATION OF PHYTOCHROMEPhotochemistry and Photobiology, 1973
- Purification of Oat and Rye PhytochromePlant Physiology, 1973
- Partial Characterization of Oat and Rye PhytochromePlant Physiology, 1973
- Phytochrome: Chemical and Physical Properties and Mechanism of ActionAnnual Review of Plant Physiology, 1972
- Chemical evidence for conformational differences between the red- and far-red-absorbing forms of oat phytochromeBiochemistry, 1972
- The Dark Reactions of Rye Phytochrome in Vivo and in VitroPlant Physiology, 1972
- “Disaggregation” of Phytochrome in Vitro—A Consequence of ProteolysisPlant Physiology, 1971
- Some Properties of Phytochrome Isolated From Dark-grown Oat Seedlings (Avena sativa L.)Plant Physiology, 1968
- Photoperiodism in PlantsScience, 1960