Compared to monovalent cations, divalent cations are very strongly retained during cation-exchange chromatography. Therefore, both mono- and divalent cations such as alkali metals and alkaline earths or mono- and diamines cannot be separated and eluted by simple isocratic chromatography. Three methods for separating and eluting mono- and divalent cations in one run are discussed in this paper: gradient elution, using an eluent step change, and column switching. For each method, the columns are packed with cation-exchange latex-coated 13-μm styrene-divinylbenzene resin. The eluents are mixtures of dilute hydrochloric acid and 2,3-diaminopropionic acid. Detection is by electrical conductivity with chemical eluent suppression.